Redox‐Modulated Speciation and Quantitative Bio-accessibility of Iron in Moringa Oleifera Leaf Extracts for Iron-Deficiency Anemia
Chemistry- Grade:
- 12
- Teacher:
- Rashmitha Kasaragod Pallath
The assessment of iron (Fe) bioaccessibility in Moringa oleifera is a critical aspect of nutritional science, offering a sustainable, plant-based alternative to synthetic iron supplements. Traditional iron pills often cause severe gastrointestinal distress and oxidative stress, highlighting the urgent need for highly bioavailable natural substitutes. This study utilizes a multi-phase analytical framework to evaluate the thermal and chemical responses of Moringa leaf matrices to optimize iron liberation. By employing a controlled drying protocol and fine powder grinding to maximize surface area, the research disrupted complex matrix-bound barriers, such as phytates, that typically inhibit iron absorption. A sequential extraction protocol featuring a hot 1 M HCl leach at 80 to 95 degrees Celsius for 60 to 90 minutes was utilized to recover acid-soluble iron oxides. Quantification was performed using both Ferrozine and Potassium Thiocyanate colorimetric assays, mapped against a 0.1 to 10 ppm calibration curve. The analysis revealed that a 50 degrees Celsius incubation process yielded the highest iron concentrations at approximately 337.4 mg/kg. This represents nearly 5.6 times more extracted iron than the air-dried samples, which yielded only 59.7 mg/kg. This significant increase demonstrates that specific thermal treatments effectively unlock plant-bound iron. Ultimately, these findings provide a robust, data-driven foundation for utilizing appropriately processed Moringa oleifera as a highly effective, functional food substitute for traditional iron therapy.
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