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Using Polymerase Chain Reaction Assays to Examine Geospatial Trends in Brown Dog Ticks Lineages Throughout Arizona

Cellular and Molecular Biology and Biochemistry
Madeleine Berry

Christine D'Antonio

With the increase of Rocky Mountain spotted fever in the Arizona region, creating a better understanding of tick movement over time will help researchers control and monitor tick and disease spread in Arizona. This research focused on creating a geospatial analysis of brown dog tick lineages in Arizona and performed a comparative analysis of previous research on tick movement. DNA was collected from 20 ticks from different counties in Arizona. PCR's were created in primers specific to the tropical and temperate lineages of ticks. The PCR's were then put through a gel to determine the lineage of the ticks tested. The results showed that a county that would have been believed to only contain temperate ticks, tested positive for a tropical tick. Results also showed that proves findings hold to the number of tropical ticks in lower counties. This research helped further scientists' knowledge on tick movement upward and further research would further field testing to learn more about tick movement.

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